Good Total PDK1 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technologies

Product Name:

PDK1 Antibody

Catalog Number:

#3062

I am investigating the role of the PDK1-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total phospho-PDK1 antibody from Cell Signaling and they are showing very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, 5% BSA, overnight, 1:1000

Blocking Agent

RT, 5% milk-TBST, 1h

Secondary Incubation

RT, 5%-milk-TBST, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad clarity

Results Summary

Very good antibody for the detection of total PDK1-Protein

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy-to-use

The Bad

None

The Bottom Line

Perfect antibody

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