BD Bioscience
Purified Mouse Anti-PKA RIIβ
610625
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Browse Similar Products: PKA Western blot Antibodies
One of the goals of my project is to carry out protein-protein interactions with components that are relevant to the hedgehog signaling. A first step in the process is to test the antibodies to determine the expression in either over-expression or endogenous levels. The goal of this experiment was to test the expression level of PKA regulatory subunit IIbeta in NIH-3T3 cells.
Western Blot
NIH-3T3 whole cell lysate
1:500 in blocking buffer and incubation at 4 deg for 16 hr on a rotator
5% Milk in 1x TBS-T (0.1 %)
1:5000 in blocking buffer and incubation at 25 deg for 1 hr on a rotator
NA
ECL
This antibody detects a band ~50 kDa in 3T3 whole cell extracts. There were no non-specific bands.
None
Specific band detection at 50 kDa and no background issues
Excellent antibody for Western Blotting