Anti-Mouse TLR3 APC for Splenocyte Staining

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD283 (TLR3) Antibody

Catalog Number:

141906

TLR3 APC was used to identify TLR3 expressing dendritic cells among murine splenocytes in different conditions (e.g. tumor bearing vs non tumor bearing mice)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes, fixed with 2% PFA, permeabilized using Saponin buffer

Primary Incubation

1:400, 30min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

Excellent antibody to identify TLR3+ DC in the spleen, easy to distinguish between positive and negative signal

Additional Notes

None

Related Categories

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Summary

The Good

Staining quality

The Bad

No complaints

The Bottom Line

Great antibody

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