Abcam
Mouse IgG1 isotype MOPC21 FITC
ab106163
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This isotope control is used in our lab for anti platelet antibody testing, where we use a monoclonal antibody to detect the presence of bound IgG to platelets in dog sample. It serves as a good isotope control.
Flow Cytometry
Whole blood in EDTA
100ul of 1:100diluted 1mg/ml isotope with the 100ul of washed cells incubated for 30mins in dark.
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FACS CAlibur
The results generated were such that we could distinguish clearly patients that have bound IgG to their platelets hence showing signs of thrombocytopenia. The isotope control helped make the difference.
None
Great Antibody
Works well