Eppendrof
Eppendrof Biophotometer D30
6133000010
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This Biophotometer from Eppendrof was used to measure protein concentration in whole cell lysate. For this, relative absorbance of protein samples with respect to standards were measured using Eppendorf's biophotometer.
Quantification of proteins and nucleic acids based on relative absorbance at fixed wavelength spectrum.
Protein samples, Protein estimation BCA reagents, Cuvettes
Cell lysate was prepared in RIPA buffer and 5microL was mixed with 1X protein estimation BCA Reagent. Solution was heated in water bath at 60 0C for 1hr to develop blue color. Similarly, standard BSA was prepared from a range of 2 to 18 microgram with a difference of 2. Absorbance of samples was measured at 562 nm wavelength in biophotometer and compared to that of standards.
None
Biophotometer had showed fast and accurate measurement of absorbance. No effect of an electromagnetic field was observed in sample readings. If the same solution was read twice, almost the same absorbance readings were observed.
Fixed wavelengths at 230 nm, 260 nm, 280 nm, 320 nm, 340 nm, 405 nm, 490 nm, 562 nm, 595 nm and 600 nm, pre-programmed features, plot graphs, defined measuring range, data storage
Fast measurement
Good biophotometer for research laboratory
Product Name: BioPhotometer D30Supplier Page