Western Blot Using Anti-CPXM2 from Proteintech Group Inc

Klin. Pharmakol. und Tox.
Charite-Berlin
PhD

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Company:

Proteintech Group Inc

Product Name:

CPXM2-Specific from Proteintech Group Inc

Catalog Number:

20082-1-AP

Browse Similar Products: CPXM2 Western blot Antibodies

We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). We performed Western blot analyses of CPXM2 protein expression in cell lysates of human PBMC using CPXM2-Specific antibodies from Proteintech Group Inc

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates of human PBMC

Primary Incubation

Dilution 1:500, 16 hours overnight, 4°C

Blocking Agent

5% Skim milk in TBST

Secondary Incubation

Dilution: 1:5000, 1 hour, room temperature

Tertiary Incubation

ECL - 5 min, room temperature

Detection

ECL detection

Results Summary

Different bands were detected after 5 min including a CPXM2-band at 86-90 kDa (see figure).

Additional Notes

None

Image Gallery

Summary

The Good

A band including CPXM2 (86-90 kDa) was detected

The Bad

Some other bands were detected

The Bottom Line

This antibody is ok for Western blot analyses of CPXM2

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