Not Specific to LXR-Beta

December 01, 2015

Microbiology
Medical College of Wisconsin
Graduate Student

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Quality of Results

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Company:

Abcam

Product Name:

Rb pAb to LXR beta

Catalog Number:

28479

This antibody recognizes both LXR alpha and LXR beta.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary BMDMs

Primary Incubation

1:1000 dilution incubated overnight at 4 degrees C

Blocking Agent

5% Milk

Secondary Incubation

1:1000 dilution of anti-rabbit HRP incubated at RT for 1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

Recognizes both LXRa and LXRb. This was validated with cells from KO mice.

Additional Notes

None

Related Categories

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Summary

The Good

OK if you are blotting for total LXR

The Bad

Not specific for beta

The Bottom Line

This antibody is not specific for LXRb. Only use this antibody if you want to detect both alpha and beta.

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