Antibody Recognizes Both Isoforms

Microbiology
Medical College of Wisconsin
Graduate Student

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Company:

Abcam

Product Name:

Rb pAb to LXR alpha

Catalog Number:

28478

The antibody was generated to recognize LXR alpha, however is also recognizes LXR beta. This was verified with cells from KO animals.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary BMDMs

Primary Incubation

1:1000 dilution incubated overnight at 4 degrees C

Blocking Agent

5% Milk

Secondary Incubation

1:1000 dilution of anti-rabbit HRP incubated at RT for 1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

This antibody is not specific for LXRa. This was verified with primary BMDMs from KO mice.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Stains something

The Bad

Stains lots of things

The Bottom Line

Non-specific antibody. Only use if you are interested in total LXR.

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