Human CRGP ELISA Kit that Works

RUSH UNIVERSITY MEDICAL CENTER
ORTHOPAEDIC SURGERY
POSTDOC

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Company:

SPI bio

Product Name:

Human CGRP enzyme imunoassay kit

Catalog Number:

A05481-96 wells

This CRGP enzyme immunometric assay kit is based on a double-antibody sandwich technique. It comes with a pre-coated 96-well plate and needed reagents. The detection range is from 7.81 to 1000 pg/ml. Protocol is the same with regular ELISA protocol.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell culture supernatant

Protocol Overview

Collect the supernatant from cell culture and follow the protocol by adding 100ul of sample into wells followed by anti-CRGP Ache tracer of 100ul. Incubate at 4C overnight and wash completely. Develop the plate and read at 414nm wavelength.

Tips

Have duplicates for samples

Results Summary

This CGRP ELISA kit works well on detecting the CGRP concentration in the cell culture supernatant.

Additional Notes

None

Image Gallery

Summary

The Good

The pre-coated plate is strip-style and easy for use.

The Bad

The shelf life of the reconstituted Ellman's reagent is limited.

The Bottom Line

This CGRP EIA kit works on cell culture supernatant with a detection range of 7.81 to 1000pg/ml.

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