Cayman Chemical Company
Substance P EIA kit
583751
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This ELISA kit assay is based on the competition between substance P and a AchE conjugate for limited amount of substance P- specific rabbit antiserum binding sites. Detection range is from 3.9pg/ml to 500pg/ml. Reading wavelength is between 405nm and 420nm.
ELISA
Rabbit cell culture supernatant
Collect the cell culture supernatant and follow the protocol by adding in the samples, substance P Ache tracer, then substance P EIA antiserum. Incubate overnight at 4C. After a complete wash, add in the Ellman's reagents and develop. Read the plate.
Make fresh Ellman's reagent every time.
This substance P ELISA kit works well for detecting the cell culture supernatant substance P concentration. The detection range is from 3.9 up to 500 pg/ml.
None
Sensitive
Some reagents only have limited shelf life after reconstitution.
This ELISA kit works on detecting the substance P concentration from rabbit cell culture supernatant.
Product Name: Substance P EIA KitSupplier Page