Excellent a2-Macroglobulin ELISA Kit

December 01, 2015

Northwestern University
Medicine, Division of Allergy-Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Alpco

Product Name:

Alpha-2-macroglobulin ELISA

Catalog Number:

30-6610

We needed a protein assay to measure a2-macroglobulin in nasal secretions, which is a biomarker for epithelial leak. This kit was easy to use, and the results were reliable. The kit only needs 10uL of sample and the wells are pre coated so the assay can be done it a day making it superior to many cumbersome ELISA kits.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human nasal secretions

Protocol Overview

Very typical ELISA protocol, except that the wells are pre-coated so the procedure can be done in 1 day.

Tips

Very straightforward

Results Summary

Reliable results, and did not need to optimize dilutions as the results fell on the standard curve.

Additional Notes

Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Sep;136(3):737-746.e4. doi: 10.1016/j.jaci.2015.01.043.

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Summary

The Good

Easy to use, reliable, better than most ELISA kits

The Bad

Not as easy as a luminex kit

The Bottom Line

If you need an ELISA kit I recommend this company, the kits are easy to use, they don't use much sample, and they take less time than more traditional ELISA kits.

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