The Good
Extremely sensitive and able to elucidate minor differences in cellular metabolism.
The Bad
Requires extensive optimization procedure, and each run could be completely different from the next...
The Bottom Line
If you're willing to put in the time and effort, you can come out with results that elucidate the extremely complex metabolic responses of cells. Money, time, and optimization aside, the final product can give you more information than several other assays at once in publication-ready graph format. This can raise more questions than it answers, in a good way.