CCR7 Expression in Human Primary PBMCs

November 24, 2015

Anaesthesiology
LMU Munich
Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-human CD197 (CCR7) Antibody

Catalog Number:

353204

Info: View Product View Product Specs

Browse Similar Products: CD197 Antibodies

In this experiment, we investigated special subtypes of T-cells, mainly the T memory cell subsets, where CCR7 expression represents an important criteria for phenotyping. This product was used for labeling of Human PBMCs in order to investigate CCR7 expression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human primary PBMCs

Primary Incubation

30 mins on ice in the dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

This antibody is suitable for investigating CCR7 expression in human primary PBMCs after Ficoll gradient centrifugation. 1 million cells, 2µl antibody, incubate as mentioned above, wash three times with FACS buffer and analyze.

Additional Notes

Compare to corresponding isotype

Related Categories

Image Gallery

Summary

The Good

Quick screening, not much antibody needs to be used, affordable, robust results

The Bad

Washing three times is a little time consuming

The Bottom Line

Nice antibody to use in a large number of cell populations, different conjugates also available.

Share your experience with other scientists. Write a Review! »

Join the discussion