Ribosomal Protein S6/RPS6 Antibody for Western blotting

October 23, 2015

Barshop Institute
UTHSCSA
Research faculty

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Ribosomal Protein S6/RPS6 Antibody

Catalog Number:

NB100-1595

This antibody was used to study the effects of a mToR inhibitor on Ribosomal Protein S6/RPS6 phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HepG2 cell lysate

Primary Incubation

1:1000 dilution overnight incubation at 4C

Blocking Agent

Odyssey Blocker in PBS (Licor)

Secondary Incubation

1:15000 IRDye 800CW conjugated goat anti-rabbit IgG for 1 h at room temp

Tertiary Incubation

NA

Detection

Odyssey infrared scanner cLX

Results Summary

The antibody showed the presence of a single band at about 30 kDa

Additional Notes

None

Related Categories

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Summary

The Good

Worked as expected

The Bad

None

The Bottom Line

This is a good antibody for detecting human and mouse Ribosomal Protein S6/RPS6

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