Good ELISA Kit for Detecting Human MMP-13 Protein

RUSH UNIVERSITY MEDICAL CENTER
ORTHOPAEDIC SURGERY
POSTDOC

Overall

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Ease-of-Optimization

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Company:

RayBiotech

Product Name:

Raybio human MMP-13 ELISA kit

Catalog Number:

ELH-MMP13

This kit works well in detecting the MMP-13 protein concentrations in the culture media of human annulus fibrosus cells. The standard curve of the kit is from 0pg/ml to 6000pg/ml which is a big range and could detect a wide range of sample concentrations. It's a precoated plate. Also the customer service of Raybiotech is really good and shippment is very efficient and only takes short time.

Experimental Design and Results Summary

Application

Cell culture media ELISA

Starting Material

None

Protocol Overview

Collect culture media and dispense 100ul of media to ELISA wells. Wash 4 times and add in biotinylated antibody for 1h and then wash. Add in streptavidin solution for 45 minutes and then wash. Add in TMB for 30min and then stop reaction. All reagents are provided by kit.

Tips

Final step of adding the stop solution, this kit required only 50ul of Stop Solution while most other kits normally need 100ul.

Results Summary

This is a good quality kit to detect human MMP-13 protein concentration in cell culture media. It works well.

Additional Notes

None

Image Gallery

Summary

The Good

Standard curve range is wide.

The Bad

Stop solution is provided with limited amount.

The Bottom Line

This kit works well in detecting the cell culture media human MMP-13 protein concentration.

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