Jackson ImmunoResearch
Biotin-SP-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (minimal cross-reaction to Human, Bovine, and Horse Serum Proteins)
115-065-062
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I have been using this secondary antibody for years in western blot and ELISA. This gives good signals with low background
Western Blot
Bacteria and yeast protein extracts
1:2000 for 1 hour
Non-fat dry milk
1:10 000 for 1hour (goat anti-mouse biotin)
1:10 000 for 1 hour (Streptavidin-HRP-
BCIP-NBT
Secondary antibody experiment easy to set-up. Good quality results. Good shelf life of the product.
None
Easy to use, good results
A bit expensive
Good product, cost-effective.