Anti Mouse CD3 PB for Flow Cytometry

October 26, 2015

Internal Medicine.
Rush University Medical Center
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Pacific Blue™ anti-mouse CD3 Antibody

Catalog Number:

100214

Info: View Product View Product Specs

Browse Similar Products: CD3 Antibodies

I used this to identify the CD3+ T Cell population in the mouse spleen to use for downstream research.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1X10^6 suspension cells were incubated with 0.5ul PB anti-mouse CD3 in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

0.5ul CD16/CD32 for 10 min at 4oC

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

The graph shows it is a very good Ab to gate CD3 population.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use.

The Bad

NA

The Bottom Line

I recommend this Ab to other colleagues.

Share your experience with other scientists. Write a Review! »

Join the discussion