DAPI Stains Cell Nuclei Clearly

Stanford
Pathology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific (Invitrogen)

Product Name:

DAPI (4',6-Diamidino-2-Phenylindole, Dilactate)

Catalog Number:

D3571

To visualize the general tissue structure and tissue organization during immunofluorescence staining, we use DAPI to stain the nuclei of all cells.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Mouse small intestine tissue section

Protocol Overview

The pre-fixed tissue section was stained with DAPI (0.5 ug/mL) for 5 minutes, washed in PBS, then mounted.

Tips

The DAPI staining solution can be reused a number of times.

Results Summary

Staining of cell nuclei is very clear.

Additional Notes

None

Related Categories

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Summary

The Good

Stains very clearly, easy to use, great for visualizing tissue structure/organization.

The Bad

None to mention.

The Bottom Line

Product is great for visualizing cells via immunofluorescence.

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