Western Blot Analysis of MFN2

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

MFN2 antibody

Catalog Number:

11925S

Info: View Product View Product Specs

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Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. In this experiment, we tried to determine the effect of treatments on the levels of mitochondrial fusion protein, MFN2, levels.

Experimental Design and Results Summary

Applications

Western Blot

Sample

PC3 cell lysates

Primary Incubation

1:3000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

We detected clear bands for MFN2 in our samples.

Additional Notes

None

Related Categories

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Summary

The Good

Very easy to detect MFN2 with this antibody compared to other sources

The Bad

None

The Bottom Line

Works very well and gives clean band

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