Good cDNA Kit

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Quanta Biosciences

Product Name:

qScript cDNA SuperMix

Catalog Number:

95048-025

This is a 1 step cDNA kit that has everything needed for cDNA synthesis in a single mix. This kit is used often to convert RNA to cDNA and I have successfully used this cDNA in downstream PCR applications.

Experimental Design and Results Summary

Application

cDNA Synthesis

Starting Material

RNA

Protocol Overview

Add desired amount of RNA with SuperMix adjusting to a final volume with nuclease free water. Follow thermocycler protocol is manual.

Tips

Follow the manual's directions

Results Summary

RNA is converted to cDNA and can be used in PCR applications with no further purification steps.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Polymerase is in the SuperMix so no way to make no RT controls.

The Bottom Line

Good kit to make cDNA.

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