Actin Antibody for Human Samples

September 14, 2015

Environ. Med.
University of Rochester
Postdoc

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Company:

Santa Cruz Biotechnology

Product Name:

Search Results Actin Antibody (I-19): sc-1616

Catalog Number:

sc-1616

Info: View Product View Product Specs

Browse Similar Products: Actin Antibodies

We had problems in identifying a good Actin antibody that works for both human and mouse samples. Based on our search for actin antibody, we found this Santa Cruz Biotechnology Ab works well as mentioned in the data sheet for both human and mouse samples. We use this Ab as our housekeeping control for all the western blots we perform in house.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human gingival fibroblast cell lysates

Primary Incubation

1:1000 in 5% milk

Blocking Agent

5% Milk for 1 hr

Secondary Incubation

1:10,000 dilution anti-goat conjugated with HRP secondary antibody

Tertiary Incubation

NA

Detection

ECL based detection

Results Summary

We found this actin antibody works well for human tissue and cell lysates

Additional Notes

None

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Summary

The Good

Very good Actin antibody for human samples

The Bad

None noted

The Bottom Line

Works both for human and mouse samples

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