eBioscience
MHC2 AF700 eBioscience
12-5322-81
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Our lab studies B-cell development in the spleen. To test activation after TLR agonist treatment, anti-MHC2 antibodies were employed. This antibody performed well and clearly distinguished B cells from T cells and detected further increases among activated B cells.
Flow Cytometry
C57BL/6 splenocytes
15-20min in FACS buffer 1:200
Fc block 5min prior
NA
Flow cytometry
This antibody showed clear separation between positive and negative expressing MHC2 cells. I would highly recommend this product to those studying immune cell activation.
None
Antibody works well at 1:200
Needs to be optimized to prevent spectral overlap with BV786
This antibody worked well with little optimization.