New England Biolabs
XbaI
R0145S
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This is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.
Restriction Digest
Plasmid DNA
Add restriction enzyme to mixture of correct NEBuffer with plasmid DNA and incubate at 37 celcius for a sufficient amount of time to digest all plasmid DNA.
Use the correct NEBuffer
The XbaI enzyme worked well cutting the vector 1 time (expected single cutter) and excised an insert in the vector that was flanked by BmtI and XbaI sites.
None
Easy to use
A good source of XbaI restriction enzyme. Another good product from NEB.
Product Name: XbaISupplier Page