Santa Cruz Biotechnology, Inc.
p38α (C-20)
sc-535
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Mammalian cells were treated with growth factor for different time points and proteins were extracted. Equal amounts of protein from each treatment were resolved and immuoblotted to check p38alpha level. This antibody from Santa Cruz Biotechnology was selected because it detects endogenous level of p38alpha in mammalian cell line.
Western Blot
Protein lysate from mammalian cell line
1:1000 (5% skimmed milk in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST
1:4000 (5%skimmed milk in TBST), 1h at RT
NA
ECL
P38alpha antibody from Santa Cruz Biotech showed excellent specificity and bright signal. However, there was no effect of growth factor in P38alpha levels.
None
Got highly specific p38alpha band
Excellent antibody for immunoblotting.