Sirt1 Expression in Neuronal Cells

September 08, 2015

Project JRF
Centre for Cellular & Molecular Biology
Postdoctoral Researcher

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Company:

EMD Millipore

Product Name:

Sirt1

Catalog Number:

09-844

Browse Similar Products: Sirt1 Antibodies

The purpose of this study was to check the expression of Sirt1 in two different cellular environments. The product has been opted after multiple comparisons and standardization.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse neural cells

Primary Incubation

1:1000 in 5% BSA dissolved in 1XTBST, incubated overnight @ 4C

Blocking Agent

5% BSA in 1X TBST, incubated 1hr @ room temp.

Secondary Incubation

Anti-rabbit from Santa Cruz, 1:5000, incubated 1hr @ room temp.

Tertiary Incubation

NA

Detection

ECL detection in Chem doc

Results Summary

Expression of Sirt1 was observed properly.

Additional Notes

None

Image Gallery

Summary

The Good

Works well

The Bad

A single non-specific band above 110 Kda can be observed.

The Bottom Line

Recommended.

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