Siglec F Antibody

Medicine
Northwestern University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Rat Anti-Mouse Siglec-F

Catalog Number:

562068

In this experiment, we were looking at eosinophils in the blood of WT and KO mice using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

0.5 ul in 100 ul, incubated for 30 minutes at 4 degrees

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR II

Results Summary

We were able to detect SiglecF + eosinophils in the blood.

Additional Notes

None

Related Categories

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Summary

The Good

It detects Siglec F

The Bad

None

The Bottom Line

This antibody is great for detecting Siglec F

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