Anti-DHFR for Westerns and IPs

July 10, 2015

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology Inc.

Product Name:

DHFR (A-4)

Catalog Number:

sc-74593

Info: View Product View Product Specs

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Mouse dehydrofolate reductase (DHFR) can be easily detected using this antibody. We analyzed samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from transgenic seedlings expressing this protein recombinantly.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves

Primary Incubation

1 to 100, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

NA

Detection

ECL

Results Summary

Works for plant extracts and for co-IPs

Additional Notes

None

Related Categories

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Summary

The Good

If optimized, good clear signal and reproducibility

The Bad

Low concentration and therefore quite expensive

The Bottom Line

Good anti-DHFR reagent for Westerns and IPs.

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