Great antibody for detecting mouse CD4 expression

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV510 Rat Anti-Mouse CD4

Catalog Number:

563106

Balb/C mice were sacrificed and isolated splencoytes were used for flow cytometric analysis . Cells were stained with anti-CD4 antibody (BV510 Rat Anti-Mouse CD4) and anti CD8 antibody. The results showed a clear CD4 positive and negative population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from mice

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on Amcyan Channel

Results Summary

Clear CD4 positive population, indicating the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Great staining. Once standardized, gives consistent and reliable results.

The Bad

Titration is required

The Bottom Line

Good replacement antibody in the Amcyan channel.

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