Santa Cruz Biotechnology, Inc.
GFP (FL)
sc-8334
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We analyze sample of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. We use this Green Fluorescent Protein (GFP) antibody to target protein fusions expressed in the plant. It needs some optimization and dilution steps to overcome initial detection problems.
Western Blot
Arabidopsis seedlings or leaves
1 to 100, overnight
4% milk TBST
1 to 2500
NA
ECL
Works okay
None
If optimized, get good clear signal and reproducibility
Low concentration
Reliable rabbit anti-GFP