Life Technologies
Occludin antibody
71-1500
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This antibody works well on differentiated human airway epithelial cells. We use at a 1:200 dilution, and store it in small aliquots at -20. Results were published in J Allergy Clin Immunololgy: Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease., Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP., J Allergy Clin Immunol. 2015 Apr 1. pii: S0091-6749(15)00188-8. doi: 10.1016/j.jaci.2015.01.043. [Epub ahead of print]
Immunofluorescence
Human airway epithelium cultured at air liquid interface
1:200 dilution, and incubate for 1h at RT or overnight at 4 degrees in PBS with 5% normal goat serum and 1% triton
PBS in 5% normal goat serum, 1% triton, 1h RT
Goat anti rabbit alexa 488 in blocking buffer
NA
Microscope analysis
Organized tight junctions could been visualized
None
Antibody works well
Required optimization for permeablization
This is a good, reliable antibody