Nice kit for gel DNA recovery

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

Zymoclean™ Gel DNA Recovery Kit

Catalog Number:

D4001

I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes.

Experimental Design and Results Summary

Application

Gel DNA Recovery

Starting Material

DNA

Protocol Overview

Cut out DNA containing piece from agarose gel. Follow Zymoclean protocol and then elute DNA from column. Verify DNA concentration and purity on Nanodrop.

Tips

Follow the manufacturer's protocol

Results Summary

After gel recovering 7 samples, the 260/230s, which I have found to be incredibly low using other gel recovery kits, were close to or above 2 for 6/7 and 1 for only a single sample.

Additional Notes

None

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Summary

The Good

Gel purifies DNA with high 260/230

The Bad

None

The Bottom Line

This is a good kit that recovers DNA with high 260/230, which means that one doesn't need to further ethanol precipitate all of the samples to remove contaminants.

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