BD Biosciences
HIF-1 alpha Antibody
610958
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This antibody is used as a control to make sure CoCl2, a hypoxia mimetic, is increasing HIF protein levels in HeLa cells.
Western Blot
HeLa cells
1:500, Overnight, 4 celcius
5% Milk in 0.1% TBS-T
HRP at 1:5000 for 1 hr at room temperature
NA
Chemilumenescence
A single band of approximately the expected size was increased with CoCl2 in HeLa cells.
None
Good antibody for detecting HIF-1 alpha
This is a good antibody for detecting HIF-1 alpha.