Purified anti-mouse CD45

Pediatrics
Pennsylvania State University College of Medicine
Postdoctoral Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Purified anti-mouse CD45

Catalog Number:

14-0451

Info: View Product View Product Specs

Browse Similar Products: CD45 Antibodies

My research work currently focuses on the relationship between influenza virus infection and type II cells in the lung. Lung type II cell isolation is kind of a complicated and long procedure. An important step to be sure of the purity of a type II cell during the isolation is incubation of digested total lung cells with an anti-mouse CD16/CD32 and CD45 coated plate for negative selection. I have used lots of antibodies from eBioscience for flow experiments before and have never been disappointed. I would purchase these purified Abs from eBioscience again to get more beautiful data. The isolated type II cells are very purified and maintained very good viability after the long procedure. I like antibody products from eBioscience because their price and quantities are competitive. I would recommend eBioscience to my colleagues.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Lung Tissue

Primary Incubation

1.5ug/million cells of anti-mouse CD45

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Abs coated plate

Results Summary

After using anti-mouse CD16/CD32 and anti-mouse CD45 pre-coated plates, high purified lung type II cells were harvested.

Additional Notes

None

Related Categories

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Summary

The Good

High performance and easy to use

The Bad

None

The Bottom Line

Overall, this purified antibody worked well for lung type II cells isolation.

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