Very Good Anti-Human PD-1 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PD-1 Antibody

Catalog Number:

558694

Browse Similar Products: PD-1 Antibodies

Human PBMC reconstituted NOD-scid IL2rγcnull mice (NSG mice), were sacrificed and single cell suspensions from the spleen was used for flow cytometry. This antibody was used for detecting PD-1 expression on human CD45 and CD3 gated T cells isolated from these humanized mice. The result showed a clear PD-1 positive population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on APC Channel

Results Summary

Clear PD-1 positive population which confirm the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Very good clean signal and reproducibility

The Bad

None

The Bottom Line

Good antibody for detecting human PD-1 expression

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