Lonza
SeaKem LE AGAROSE 500G
50004
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We routinely use this agarose to run DNA gels, it melts easily and I have made gels as low as 0.7% as well as very thin gels that hold up to the heat of running gels at up to 200V. The gels also show even migration throughout the gel.
Running agarose DNA gels
Various
Add the desired amount of agarose for your % gel in 1X TAE buffer and then melt via microwave. After letting it cool until it is slightly warm in your hand, pour onto DNA mold and let set. Load samples and run gel.
Make sure agarose is fully dissolved in TAE or gels will run weird.
Running multiple colony PCR samples and a DNA ladder on a gel yields excellent results with the colony PCR samples migrating the same as the positive control (2nd lane) and the DNA ladder showing a size between 2000 and 3000 bp (expected size ~2600 bp). The gel also had very low background signal letting the bands can be easily viewed.
None
Makes a good agarose gel
This is good agarose and I would recommend it.
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