Life Technologies
Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
A-11055
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I was using this secondary antibody to detect a primary goat anti-FOXJ1 antibody in primary human bronchial epithelial cells. Immunofluorescence shows strong nuclear staining as expected.
Immunofluorescence
Primary Human Epithelial Cells
Anti-goat FOXJ1, Overnight, 4 celcius
3% BSA
1:500, 1 hour, Goat anti-donkey 488
NA
Confocal Fluorescent Microscopy
Confocal image shows nuclear 488 signal, which is where I expect the FOXJ1 goat primary antibody to bind.
None
It detects goat primary antibodies
This is a good secondary and I would recommend it.