Make sure you get the HS (high sensitive) kit

April 07, 2015

University of Wisconsin-Madison
Department of Food Science
Research Assistant

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Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human IL-6 Quantikine HS ELISA Kit

Catalog Number:

SS600B

I am working on an intervention study to look at whether yogurt consumption can reduce chronic inflammation, which is linked to the development of various diseases. Il-6 has been used as biomarker for inflammatory levels in numerous reports.

Experimental Design and Results Summary

Application

To determine human plamsa levels of IL-6

Starting Material

Heathy human EDTA plasma

Protocol Overview

1) Bring samples and kit to room temperature. 2) Prepare the standard stock solution. It takes a minimum of 15 mins for the standard to dissolve; so plan ahead for that. Be advised: for healthy human plasma, there is no need to dilute the plasma samples. 3) Add dilution buffer to the plate (do not forget this step!) 4) Follow the kit insert: load samples, control and standard-----incubate on orbital shaker---wash---add conjugate--incubate on orbital shaker--wash----add substrate---add amplifer---add stop solution--read (takes about 6-7 hours due to the long incubation period)

Tips

Prepare standard stock solution, substrate solution and amplifier solutions ahead.

Results Summary

The kit works pretty well. I got acceptable cvs within and between batches.

Additional Notes

None

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Summary

The Good

Works reliably; Generous with the volume of reagents (I usually save the leftover reagent for emergency use; make sure the same batch)

The Bad

A little pricey.

The Bottom Line

I will buy this kit again. If you have a lot samples, talk with the sale reps. They are usually very nice and can offer you some discount.

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