Cell Signaling Technology
VEGF Receptor 2 Antibody
2472
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This antibody was used in a study that used VEGFR2 as a marker for endothelial cell (EC) differentiation from MSCs. Several antibodies and concentrations were tried, but ultimately Cell Signaling's #2472 gave the best results for IF visualization of VEGFR2.
Immunofluorescence
Normal human-derived endothelial cells grown on a cell culture slide
1:200 in blocking solution overnight at 4°C
5% BSA + 0.3% Triton X-100 in 1x PBS
5 ug/mL Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate (Life Technologies cat #A-11037) in blocking solution for 1 hour at room temperature
Endothelial cells also stained with DAPI (1:5000 of 5 mg/mL stock in 1x PBS for 3 minutes at room temperature) for nuclear localization
Nikon Eclipse Ti Microscope with fluorescent filters, 20x objective
Cell Signaling's VEGFR2 antibody worked well for immunofluorescence antibodies when paired with an AlexaFluor-conjugated secondary. Minimal background and non-specific binding was observed. Specific for cells expressing VEGFR2 when compared to non-differentiated MSCs and a non-VEGFR2 expressing control.
None
Specific antibody with minimal background.
May require some optimization to achieve best results.
A solid antibody for observing VEGFR2 expression by immunofluorescence.