Immunohistochemistry-Paraffin

Environ. Med.
University of Rochester
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Rev-erb alpha

Catalog Number:

H00009572-M02

Our work focuses on changes in Nr1d1 protein expression in human and mouse lungs. From the available source of antibody we identified this mouse monoclonal antibody for Rev-erb alpha to try in both mouse and human lung tissues for IHC.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human lung

Primary Incubation

Overnight incubation with primary antibody 1:250

Blocking Agent

10% Goat serum

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

NA

Detection

DAB

Results Summary

Rev-erb alpha positive staining in different lung cell types.

Additional Notes

None

Image Gallery

Summary

The Good

Good for IHC but not for WB

The Bad

Too many non-specific bands when used in WB.

The Bottom Line

Recommend for IHC in paraffin embeded tissue sections

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