Abcam
Anti-CD68 antibody [ED1]
ab31630
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Our research investigates inflammation during bladder regeneration. Macrophages are one of the inflammatory cells we study. We were trying to find a specific antibody to detect macrophages in rat tissue. CD68 is a marker for macrophages. The reason we chose this antibody is that this antibody is a monoclonal antibody used for immunohistochemistry and can be used in multiple species, including rat. Also, it produces lots of data images to ensure the quality of the antibody.
Immunohistochemistry
Rat bladder tissue
1:800 in 1% BSA, 4C overnight
10% goat serum in 1x TBS
1:400 at room temperature, 1 hour
Strep HRP 1:400 at room temp for 30 minutes
DAB 2 minutes
Good staining of macrophage cells.
None
Good contrast, no background, sensitive
Not 100 % specific on macrophages
Nice IHC staining for macrophages in rat tissue without any background.