Tubulin beta-III for Immunocytochemistry

March 04, 2015

chemical biology
indian institute of chemical technology

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Company:

EMD Millipore

Product Name:

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

Catalog Number:

MAB1637

This product was used to stain the differentiated neuroblastoma cells (N2A cells) to study the differential neurite out growth after treating with neurotrophic compounds.

Experimental Design and Results Summary

Applications

Immunocytochemistry

Sample

N2A cells (Mouse Neuroblastoma)

Primary Incubation

1:200 in 2% BSA of 1xPBST(0.1% Tritonx-100), four degrees overnight

Blocking Agent

2% BSA in 1xPBST(0.1% Tritonx-100), 2 hours Room temperature

Secondary Incubation

Anti-mouse-Alexa 488 1:400, 90 minutes room temperature

Tertiary Incubation

NA

Detection

Flourescent microscope

Results Summary

The differentiated N2A cells stained with the tubulin-beta-III antibody

Additional Notes

None

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Summary

The Good

The neurites of differentiated N2A cells can be stained

The Bad

Standardization of antibody dilution

The Bottom Line

Recommended for use

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