Easy to Use Beads for Compensation

University of Virginia
Neuroscience
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

UltraComp eBeads

Catalog Number:

01-2222-42

These are single stained beads for compensation control. These beads are great for compensation. We use 5ul/antibody for making the compensation per sample and the signal is still very strong.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Macrophages

Protocol Overview

5ul beads, 10 minutes incubation in 100ul FACS buffer with antibody, 2 wash

Tips

If your flow machine has a discriminator setting make sure it's set to 50 or 0 as the beads will not show up at higher discriminator settings

Results Summary

Excellent peak separation. If using cells with high background, like macrophages, set the voltages lower than you'd expect for the compensation.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Can't use with high background starting material for compensation (e.g. myelin)

The Bottom Line

Highly recommended!

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