Semi-dry Western blot

indian institute of chemical technology
chemical biology

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

Pierce G2 fast blotter

Catalog Number:

88217

This product is used to transfer proteins from sds-page gel to PVDF membranes of 45kda and ~14 kda proteins. Compared to wet transfer it reduces the time (15min 20v), consumes less transfer buffer (40ml/gel from Thermo), and is more user friendly.

Experimental Design and Results Summary

Tips

Standardize the voltage and time as per the protein molecular weight which needs to be transferred.

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Summary

The Good

Saves time, consumes less buffer, user friendly.

The Bad

Standardize the temperature and time as per the protein to be transferred and no time and voltage display.

The Bottom Line

Time-saving for people who regularly do Western blot.

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