Cell Signaling Technology
Phospho-Vimentin (Ser56) Antibody
3877P
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Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin because it provides us novel insights into its regulation at different compartments. Vimentin is known to be phosphorylated at various motifs; however there are very few antibodies available to detect them on Western blot. Here we test a Ser56 clone.
Western Blot
Mouse cell lines
1:100 BSA
5% BSA
1:1000 HRP
NA
FACScan
From the detection it can be seen that only higher oligomers are detectable although the samples are denatured. No bands at 54kDa are seen, which remains unexplainable.
None
Very clean antibody
Only oligomeric detection
Need to test another antibody or other cell lines to confirm the specificity.