Millipore
Anti-tyrosine hydroxylase rabbit pAb
657012
Info: View Product View Product Specs
Browse Similar Products: Anti-Tyrosine Hydroxylase Antibodies
This antibody is used to look for dopaminergic neurons within the SNpc and their termini within the striatum using the MPTP-mouse model of Parkinson’s disease. Following staining, tissues are mounted on slides and neurons quantified using stereological analysis and striatal density quantified using ImageJ. We used this antibody to stain fresh frozen tissue to look at SNpc. Works well with DAB staining - little to no non-specific binding.
Immunohistochemistry
Brain tissue
1:2000
5% NDS
1:200, 1 hour, room temperature
NA
DAB
Staining is always very good. It is specific to neurons we are interested in with good staining, even with thicker tissue sections (30 microns).
None
Consistent
This is the antibody we have used for years and years when doing DAB staining. No plans to switch anytime soon.