Solid Antibody for Detecting Endogenous mTOR

May 09, 2014

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Molecular Biology
University of Houston
Graduate Student

Company:

Cell Signaling

Product Name:

mTOR Antibody

Catalog Number:

2972

We use Cell Signaling's Rabbit mTOR antibody repeatedly in our lab's work studying the mTOR signaling pathway and its role as a target in biologically directed therapy. This antibody sees a lot of use in our lab and we require consistency and sensitivity over projects that may last years.

The images shown are for an siRNA knockdown screening where we were using the mTOR antibody to evaluate knockdown of mTOR expression at 72 hours post-transfection.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates for siRNA knockdown screen

Primary Incubation

1:1000, O/N @ 4C

Blocking Agent

StartingBlock T20 for 20 minutes @ RT

Secondary Incubation

Anti-rabbit 1:5000, 60 minutes @ RT

Tertiary Incubation

None

Detection

Supersignal luminol/peroxide fluorescence on film

Results Summary

Demonstration of successful mTOR knockdown by both mTOR siRNAs #1 and #2 when compared to all controls (untransfected cell line, transfection reagent only, and scrambled siRNA).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Consistent, highly sensitive and specific antibody with good purity/clean imaging results

The Bad

None

The Bottom Line

Cell Signaling's rabbit mTOR antibody is a reasonably priced, well functioning antibody that sees repeated use across several projects in our laboratory.

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