Abcam
Anti-BRDT antibody
ab5157
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Browse Similar Products: BrDT Immunofluorescence Antibodies
Our research addresses reproduction and infertility. This antibody was used to detect BRDT in mouse testis. Paraffin sections were used. As per the data sheet, this antibody was proposed to work in mouse samples, but has not been tried previously. HIER procedure was followed for antigen retrieval using 0.1m citrate buffer at pH 6. 1.500 dilution worked effectively with no background. Immunofluorescence procedure using FITC conjugated donkey anti rabbit secondary was used for labeling. It worked beautifully.
Immunofluorescence
Mouse testis parafin section
1.500 dilution, overnight incubation at 4c
10% donkey serum, 1hr at room temp
1.500 dilution at room temperature for 45 minutes
None
Fluorescence
BrDT is a nuclear protein associated with chromatin. It is expressed in the nucleus of spermatocytes in each tubule. Negative control without exposure to primary antibody indicates the specificity of this antibody.
Excellent antibody for mouse tissues.
I have not encountered anything bad about this product
I took the risk of trying the antibody in mouse sections for IF and I am glad I did 'cos I got good staining.