Advanced Biotechnologies Inc
Toxoplasma gondii RH Strain Quantitated DNA
08-948-250
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I am using this quantitated T.gondii DNA in my undergraduate thesis research, as part of a PCR protocol that will detect the presence of the parasite in various mammals collected in Western North Carolina. This specific product was chosen because this was quantitated, which will help me to gauge the amount of T.gondii in my specimen. I also was trying to keep a tight budget, and this was definitely the best buy for the amount and price of the product.
In the image, these are the product bands at various concentrations from 1.1X10^4 copies/uL at lane 2 (orig concentration) down to 1.1x10^-1 copies/uL at lane 8.
PCR
N/A
The product was used as a positive control in a series of PCR reactions on heart and brain tissues.
Would recommend separating the DNA into aliquots so as not to freeze/thaw the DNA more than is necessary.
Produced a gradient of DNA dilutions in PCR reactions and gel electrophoresis, to compare with my samples. The bands came out very thick and bright, even at a 1.1 x 10^2 dilution. I also performed an annealing temperature gradient, with B1 gene primers, from 45C to 57C and all 12 bands came out bright.
None
Easy to optimize, bright/thick bands.
No complaints here!
Very good product, easy to use (so easy an undergrad can do it), fair price.
Product Name: Toxoplasma gondii RH Strain Quantitated DNASupplier Page