Great Staining and Separation

December 26, 2013

Overall

Quality of Results

Ease-of-Optimization

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Microbiology and Immunology
University of North Carolina at Chapel Hill
Graduate Student

Company:

Biolegend

Product Name:

PE/Cy7 anti-human CD11b Antibody

Catalog Number:

301321

Trying to isolate CD11b+ macrophages and neutrophils, great for separation upon FACS and for sorting.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:400, 30 minutes, 4°C

Blocking Agent

10% FCS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Great separation. Use in all panels

Additional Notes

None

Related Categories

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Summary

The Good

Great separation. Always in stock

The Bad

None

The Bottom Line

Buy it for you panels with CD11b

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