Life Technologies
β-Galactosidase, Rabbit IgG Antibody Fraction
A-11132
Info: View Product View Product Specs
Conditional KO mouse is a very useful tool to analyze gen and protein function in vivo.Tamoxifen is one of the most usual methods to induces Cre expression to knockdown gen expression.One of the problems of the technique is the ability of tamoxifen mollecule to reach to all the somatic cells of the mice. Co-expression of b-Gal under Tamoxifen promoter allow us to determine which cell has delete our gene of interest.
Immunohistochemistry
Mice 3months after Tamoxifen treatment
1/500
1%FBS 1%BSA in TBS-T
Leyca Powervision
Not needed
DAB
Cellular and nuclear localization of b-Gal protein. Mosaic is due of the penetrance of Tamoxifen to each cell.
Retrieval: citrate buffer (pH 6)
Specific signal. strong signal
Any remarkable.
Mosaic is due to different level of induction of tamoxifen promoter to each cell.